Key Findings
Automated ATF perfusion guided by real-time metabolic control significantly intensifies cell production compared to traditional methods. In a case study involving NK cell production at TU Wien, this approach achieved a viable cell density more than three times higher than that of fed-batch processes while maintaining high viability and utilizing a small working volume.
Technical Details
The system replaces periodic sampling with continuous, real-time measurements of glucose and lactate. These metabolic data points are used to automatically adjust perfusion parameters throughout the expansion phase. Specifically, the current metabolic conditions dictate the feed and permeate rates, allowing the system to respond immediately to metabolic shifts.
Background & Context
Standard batch and fed-batch processes often limit the maximum achievable viable cell density. Furthermore, reliance on periodic sampling creates a time lag that may prevent operators from detecting metabolic shifts quickly enough to implement meaningful interventions. The integration of real-time monitoring with ATF perfusion addresses these limitations by creating a more responsive and adaptive culture environment.
Significance & Outlook
The ability to compare batch, fed-batch, and controlled perfusion performance side-by-side highlights the advantages of adaptive cell culture control. By utilizing real-time metabolic monitoring to guide automated hardware, producers can intensify processes and optimize cell density without sacrificing viability.
Get our weekly technology intelligence — free
Receive an infographic that lets you judge at a glance whether each field’s analysis report is worth reading.
Subscribe Free — Weekly Tech Intelligence
By subscribing, you’ll receive Troy-Technical’s weekly technology intelligence newsletter.
- Your email and selected fields are used only to deliver the newsletter.
- We never share your information with third parties.
- You can unsubscribe anytime via the link in each email.
See our Privacy Policy for details.
Takes about a minute · Unsubscribe anytime
